Comparison of Antimicrobial Activity of Root Canal Sealers: An In Vitro Study
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Abstract
Background and objective: The antimicrobial activity of root canal sealers may contribute to the eradication of remaining microbes in root canal. The aim of this study was to investigate antimicrobial effect of bioceramic sealer (NeoSEALER® Flo), resin sealer (ADSEAL Plus), and MTA based sealer (MTA- Fillapex).
Materials and methods: The present study aimed to comparatively verify the sealers antimicrobial activity against microorganisms. Enterococcus faecalis (E.faecalis) and Candida albicans (C.albicans) were submitted to the agar diffusion test (ADT) and modified direct contact test (MDCT), at 1hour, 1day, and 1week interval.
Results: In ADT, zone of inhibition (ZOI) were measured in millimeters. In MDCT, bacterial suspensions were exposed to the sealers, and colony-forming unit (CFU) counts were recorded to assess microbial survival. In ADT, At the 1hour and 1week interval, all sealers demonstrated no antibacterial activity against E.faecalis (ZOI=0.0mm), its only observed at the 1day, with NeoSEALER® Flo exhibiting the strongest activity (ZOI=13.19±0.37), followed by MTA Fillapex (12.19±0.37) and ADSEAL Plus (8.81±0.37). In MDCT, NeoSEALER® Flo showed higher antibacterial properties, decreased at 1week interval, followed by MTA Fillapex and ADSEAL Plus. In ADT and MDCT, MTA Fillapex showed highest and most consistent antifungal activity across all intervals and increased with time interval, followed by NeoSEALER® Flo, except for ADSEAL Plus, decreased with time interval.
Conclusion: All sealers showed antifungal activity against C.albicans at all intervals, none of the sealers maintained antibacterial activity against E.faecalis after 1week. Indicated a temporary antibacterial effect, which diminishes over time
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References
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