In vitro anticancer activity of methanolic extracts, non-thermal plasma and iron oxide nanoparticles on human cancer cell line [HepG2]
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Abstract
Introduction: Liver cancer is one of the most common malignancies across the globe and one of the major causes of cancer-related mortality. With limited available treatment options, there is an urgent need to look for new available options. Medicinal plants is an important and has been shown to possess tremendous pharmacological potential. The objective of the present study was therefore to evaluate the anticancer effect of the Passiflora incarnata and Arctium lappa Leaves compared with non-thermal plasma and iron oxide nanoparticles.
Methods: In this study determination of viability carried out through MTT assay, also molecular techniques were carried and proteomics assay of genes (P53 and Bcl2) compared with β-actin gene as standard.
Results: MTT assay revealed that P. incarnata and A. lappa leaves extracts reduced the cell viability of HepG2 cancer cells from 100 to 85 and 80%, respectively in a dose-dependent manner (20 0µl). The results of this study proved that, there were a significant killing effect for extracts, gliding Arc plasma exposure and nanoparticles. These results supported by analysis of genes associated with tumor which were P53 as tumor suppressor gene and Bcl2 (Bcl-xl) as antiapoptotic gene. Non-thermal plasma it had effects on increased transcription and protein product leading it increase in apoptotic effect of HEPG2 cell line at 40, 60 and 80sec, which were 74.33±1.07, 82.53±1.15 and 88.05±0.36%, respectively. Regarding the effect of methanolic extract of P. incarnata and A. lappa leaves. This study illustrated that, P. incarnata and A. lappa (group5 and group6), it has high effects on increased gene transcription P53 compared control (group1), which were 99.89±0.40 and 98.07±1.08%, while the result of iron oxide nanoparticles (group7), was 99.76±1.10%, compared with control group, which was 66.22±2.08%, respectively
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References
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